How do you perform a cytology smear?

How is cytology performed?

Scrape or brush cytology: This procedure involves scraping or brushing some cells from the organ or tissue that’s being tested. Some areas where doctors use scrape or brush cytology include the breathing tubes that lead to the lungs, cervix (for a Pap test), esophagus, mouth and stomach.

How is smear prepared for cytology?

The scrape cytology smears were prepared by scraping the freshly cut surface of the lesion with one end of a glass slide or scalpel blade. The accumulated scraped material was spread between two glass slides in a manner similar to the preparation of bone marrow smears, as de- scribed previously.

How do you make a cytology slide?

The staining procedure:

  1. Make sure the sample on the slide is dry.
  2. Dip the slide in each jar between six-10 times (10-15 seconds in each solution). …
  3. Wash the slide with water after jar number 3 ONLY.
  4. Dip the slide in the water jar.
  5. Never wash the slide in between the staining process.
  6. Alternative to Jar 4:
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What are the two types of cytology?

There are two main kinds, or branches, of cytology: exfoliative cytology and intervention cytology. Healthcare providers can use cytology tests for almost all areas of your body.

How long does a urine cytology take?

The procedure takes between 10 and 20 minutes. Because the urine from your first morning urination remains in your bladder for many hours through the night, the cells may degrade and not be useful for urine cytology.

What fixatives can be used for cytology smears?

Ethyl alcohol (95%) is the most commonly used fixative in cytology. The cytology samples are processed commonly by direct smear, centrifugation, cytocentrifugation, liquid-based preparation, Millipore technique and cell block.

Why is fixation of cytology specimens necessary?

Immediate fixation of cytology specimens is critical to the preservation of the cellular components. It is important that no air-drying occurs prior to fixation. If a smear is already air- dried it should NOT be put in alcohol fixative.

Do you heat fix cytology slides?

To preserve the integrity of the cells, do not heat fix these slides. Once the slide sample is dry, stain using the three-part Diff-Quik stain.

Is cytology a smear?

A cervical cytology (cervical smear) sample is a sample of cells from the outside of the cervix (or neck of the womb) that allows detection of pre-cancerous abnormalities of the cervix. These changes can then be treated successfully before cancer develops.

What is called cytology?

Cytology is the study of individual cells of the body, as opposed to histology which is the study of whole human tissue itself.

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What are the types of cytology?

There are two methods of collecting cells for cytopathologic analysis: exfoliative cytology, and intervention cytology.

  • Exfoliative cytology.
  • Intervention cytology.
  • Imprint cytology.

What are the stains used in cytology?

The universal stain for cytological preparations is the Papanicolaou stain. Harris’ hematoxylin is the optimum nuclear stain and the combination of OG6 and EA50 give the subtle range of green, blue and pink hues to the cell cytoplasm.

What is fixation in cytology?

In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing biochemical reactions and may also increase the treated tissues’ mechanical strength or stability.

How do you prepare for a slide for a Pap smear?

Rotate a spatula 360° once to obtain a single sample. Smear the sample onto the labeled slide. Fix the sample immediately (before it is air-dried) using a cytology spray fixative. Hold the fixative 15-20 cm (6 to 8 inches) away from the slide and evenly spray the slide by depressing the plunger 2 or 3 times.